The computers do not know tissue from glass.  Methods must be used to identify image regions (tiles) that contain tissue.

Our efforts makes use of the excellent work: https://github.com/deroneriksson/python-wsi-preprocessing

Process:

-Apply filters for tissue segmentation

-Mask out non-tissue by setting non-tissue pixels to 0 for their red, green, and blue channels. 

-This algorithm includes a green channel mask, a grays mask, a red pen mask, a green pen mask, and a blue pen mask.

-Threshold mechanisms: basic, hysteresis, and Otsu 

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