The computers do not know tissue from glass. Methods must be used to identify image regions (tiles) that contain tissue.
Our efforts makes use of the excellent work: https://github.com/deroneriksson/python-wsi-preprocessing
Process:
-Apply filters for tissue segmentation
-Mask out non-tissue by setting non-tissue pixels to 0 for their red, green, and blue channels.
-This algorithm includes a green channel mask, a grays mask, a red pen mask, a green pen mask, and a blue pen mask.
-Threshold mechanisms: basic, hysteresis, and Otsu